Research News

Maobing Tu | Department of Biomedical, Chemical, and Environmental Engineering

Phenolic compounds significantly inhibit microbial fermentation of biomass hydrolysates. To understand the quantitative structure-inhibition relationship of phenolic aldehydes on alcoholic fermentation, the effect of eleven different substituted benzaldehydes on the final ethanol yield was examined. The results showed that the degree of phenolic benzaldehydes inhibition was strongly associated to the position of phenolic hydroxyl groups, but not the number of phenolic hydroxyl groups. It was observed that ortho-substituted 2-hydroxybenzaldehyde resulted in 15-20 fold higher inhibition than the meta- or para-substituted analogues of 3- and 4-hydroxybenzaldehydes. By correlating the molecular descriptors to inhibition potency in yeast fermentation, we found a strong relationship between log P (octanol/water partition coefficient) of aldehydes and EC50 value. The most inhibitory 2-hydroxybenzaldehyde has a highest log P and possesses an ortho –OH group capable of forming an intramolecular hydrogen bond, which can potentially increase the cell membrane permeability and their toxicity. The results also indicated that the calculated free energy change between phenolic aldehydes and amino acids can be used to predict their structure-inhibitory activity relationship.


Cited from Dr. Tu's Publication, “Substituent Effect of Phenolic Aldehydes Inhibition on Alcoholic Fermentation by Saccharomyces cerevisiae”


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